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TotalReCaller | Improved accuracy and performance via integrated alignment and base-calling

A base-caller which opens new avenues for reducing errors significantly in short sequencing reads simply by injecting knowledge from a reference source genome through a base-by-base alignment algorithm. The performance of TOTALRECALLER leads to the conclusion that, by using a Bayesian approach that relies on dynamically creating a reference-based prior, it is possible to significantly lower the error rates of Illumina short reads for both small and large genomes, independent of the sequencing technology used (GAI or GAIIx).

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TotalReCaller forum

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TotalReCaller classification

TotalReCaller specifications

Unique identifier:
OMICS_02136
Interface:
Command line interface
Input format:
INT, CIF
Operating system:
Unix/Linux
Stability:
Stable
Software type:
Package/Module
Restrictions to use:
None
Biological technology:
Illumina
Computer skills:
Advanced
Maintained:
Yes

TotalReCaller distribution

versioning

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No versioning.

TotalReCaller support

Maintainer

  • Fabian Menges <>

Credits

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Publications

Institution(s)

Computer Science Department, Courant Institute, New York University, NY, USA; Quantitative Biology Center, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA

Funding source(s)

Grant from NSF CDI program and Abraxis BioScience, LLC

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