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RBS Calculator / Ribosome Binding Site Calculator

Controls translation initiation and protein expression in bacteria. RBS Calculator is a predictive design method that: (i) predicts the rate of translation initiation for every start codon in an mRNA transcript in its reverse engineering mode, and (ii) optimizes a synthetic RBS sequence to achieve a targeted translation initiation rate in its forward engineering mode. This method employs a thermodynamic model of bacterial translation initiation to calculate the Gibbs free energy of ribosome binding.


Predicts the translation efficiency of existing mRNA sequences and designs synthetic ribosome binding sites (RBSs) for a given coding sequence (CDS) to yield a desired level of protein expression. The synthetic RBS could be used to maximize translation efficiency for the intensive production of therapeutics or industrial proteins. It may assist in balancing the levels of enzymes in a synthetic metabolic pathway to optimize the metabolic flux, or it could be used to optimize protein expression of components in constructed genetic circuits to ensure robust operation.