A collection of tools for downstream analysis of Rep-Seq data, including clustering and phylogenetic analysis. TRigS assists with the determination and analysis of B-cell lineage trees from next-generation sequencing data. TRigS consists in various tools, including 1) AnnotateTree creates annotated lineage trees and sequence alignments showing the point at which amino acid substitutions occur 2) RevertToGermline uses a simple approach to infer the germline ancestor of a B-cell variable region sequence, given the IMGT junction analysis. 3) Clustering tools support the clustering of sequences for clonal analysis, and their large-scale depiction. 4) Tools for junction parsing and results manipluation support the integration of IgBLAST into an IMGT-style pipeline, and ease the processing of tab-separated analysis files.

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TRIgS classification

TRIgS specifications

Software type:
Toolkit
Restrictions to use:
None
Programming languages:
Python
First release date:
2015-07-01
Requirements:
Python 2.7+
Interface:
Command line interface
Operating system:
Unix/Linux
Computer skills:
Advanced
Stability:
Stable

TRIgS support

Documentation

Maintainer

Credits

Publications

  • (Lees and Sheperd, 2015) Utilities for High-Throughput Analysis of B-Cell Clonal Lineages. Journal of Immunology Research.
    DOI: 10.1155/2015/323506

Institution(s)

Institute of Structural and Molecular Biology, Birkbeck College, University of London, Malet Street, London, UK

Link to literature

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