A collection of tools for downstream analysis of Rep-Seq data, including clustering and phylogenetic analysis. TRigS assists with the determination and analysis of B-cell lineage trees from next-generation sequencing data. TRigS consists in various tools, including 1) AnnotateTree creates annotated lineage trees and sequence alignments showing the point at which amino acid substitutions occur 2) RevertToGermline uses a simple approach to infer the germline ancestor of a B-cell variable region sequence, given the IMGT junction analysis. 3) Clustering tools support the clustering of sequences for clonal analysis, and their large-scale depiction. 4) Tools for junction parsing and results manipluation support the integration of IgBLAST into an IMGT-style pipeline, and ease the processing of tab-separated analysis files.
