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UCHIME | Improves sensitivity and speed of chimera detection

A package that detects chimeric sequences from PCR with two or more segments, avoiding to interpret them as novel species. UCHIME uses a database containing no chimera-sequences or detects chimeras de novo by exploiting abundance data. This software displays a better sensitivity than ChimeraSlayer, which was the previous most sensitive detection method.

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UCHIME classification

UCHIME specifications

Unique identifier:
Command line interface
Input data:
Reference database of parent sequences provided by the use, or query ADN or ARN
Operating system:
Computer skills:
Software type:
Restrictions to use:
Output data:
chimeric alignment
Programming languages:
Source code URL:

UCHIME distribution


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UCHIME support



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Tiburon, CA, USA; Genome Sequencing and Analysis Program, The Broad Institute, Cambridge, UK; Department of Chemistry and Biochemistry, University of Colorado, CO, USA; School of Engineering, University of Glasgow, Glasgow, UK

Funding source(s)

This package was funded by the National Institutes of Health (grant U54-HG004969), the Engineering and Physical Sciences Research Council Career Acceleration Fellowship (EP/H003851/1) and the National Institutes of Health (grants HG004872 and HHMI).

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.