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uMELT specifications


Unique identifier OMICS_05317
Name uMELT
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for uMELT

uMELT citations


CtGEM typing: Discrimination of Chlamydia trachomatis ocular and urogenital strains and major evolutionary lineages by high resolution melting analysis of two amplified DNA fragments

PLoS One
PMCID: 5892870
PMID: 29634761
DOI: 10.1371/journal.pone.0195454

[…] change the melting domain structure. Further, there was complete concordance between our observations of early melt domain magnitude and presence/absence, and in silico prediction using the software uMELT [] (data not shown).A minor limitation of the study is that we have not fully defined the ability of CtGEM typing to identify C. trachomatis LGV strains. We experimentally established that the o […]


Distinction of Plasmodium ovale wallikeri and Plasmodium ovale curtisi using quantitative Polymerase Chain Reaction with High Resolution Melting revelation

Sci Rep
PMCID: 5762660
PMID: 29321578
DOI: 10.1038/s41598-017-18026-1

[…] end, we first aligned the PCR products from the in silico PCR, using the multialin online tool ( Then, we performed a melting temperature simulation, using uMELT online tool (available at, to assess if the differences observed in the PCR products would be sufficient or not to distinguish Poc and Pow using HRM detec […]


Gaussian decomposition of high resolution melt curve derivatives for measuring genome editing efficiency

PLoS One
PMCID: 5754072
PMID: 29300734
DOI: 10.1371/journal.pone.0190192

[…] s (period = 2) are shown in (blue traces). Again, the initial AT-rich region exhibited lower free energies (∆Gs) for F8-S2 sequence than that of the CCR5 sequence ().We next used the online web tool uMelt [] to determine if the melting profiles of F8-S2 and CCR5 amplicon sequences could be distinguished by in silico analysis. For F8-S2 amplicon, the derivative melt curve predicted by uMelt web to […]


Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real Time PCR

PMCID: 5788046
PMID: 29372257
DOI: 10.1167/iovs.17-22597
call_split See protocol

[…] ed for coding stretches that would yield PCR products with distinct, predicted melting curve profiles. Such amplification targets were identified in silico using the melting curve prediction software uMelt. Predictions were made based on thermodynamic parameters described by Blake and Delcourt, assuming default concentrations of monovalent ions (20 mM), free magnesium (3 mM), and a temperature ste […]


A Novel Multiplex HRM Assay to Detect Clopidogrel Resistance

Sci Rep
PMCID: 5700108
PMID: 29167499
DOI: 10.1038/s41598-017-16310-8

[…] esign of amplicons that could amplify in the same tube and melt within distinct temperature intervals to permit genotyping by multiplex HRM. Genotyping by multiplex HRM was used on clinical targets–. uMelt software was used as a tool to predict multiplex melting temperatures. In the initial triplex PCR design, the ΔTm between rs4986893 and rs12248560 was only 1 °C. Increasing the length of the amp […]


Derivation of nearest neighbor DNA parameters in magnesium from single molecule experiments

Nucleic Acids Res
PMCID: 5728412
PMID: 29177444
DOI: 10.1093/nar/gkx1161

[…] (). Statistical data analysis allowed us to extract the NNBP energies with 0.1 kcal/mol precision over two orders of magnitude of monovalent salt concentration. These values are currently used by the uMelt server to predict melting temperatures. ( ().Force techniques present several advantages compared to bulk methods (e.g. calorimetry, UV absorbance, fluo […]


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uMELT institution(s)
School of Computing, University of Utah, Salt Lake City, UT, USA

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