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uMELT specifications


Unique identifier OMICS_05317
Name uMELT
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for uMELT

uMELT citations


Distinction of Plasmodium ovale wallikeri and Plasmodium ovale curtisi using quantitative Polymerase Chain Reaction with High Resolution Melting revelation

Sci Rep
PMCID: 5762660
PMID: 29321578
DOI: 10.1038/s41598-017-18026-1

[…] for poc and pow respectively. as a sequence variation in predictive pcr product for poc and pow was observed, hrm reaction could highlight a difference between both species. using the specific umelt online tool for pcr product melting temperature (tm) analysis, predicting melting temperature from the hrm phase based on an algorithm analyzing dna bounds strength, tm could be predicted […]


Gaussian decomposition of high resolution melt curve derivatives for measuring genome editing efficiency

PLoS One
PMCID: 5754072
PMID: 29300734
DOI: 10.1371/journal.pone.0190192

[…] = 2) are shown in (blue traces). again, the initial at-rich region exhibited lower free energies (∆gs) for f8-s2 sequence than that of the ccr5 sequence ()., we next used the online web tool umelt [] to determine if the melting profiles of f8-s2 and ccr5 amplicon sequences could be distinguished by in silico analysis. for f8-s2 amplicon, the derivative melt curve predicted by umelt web […]


Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real Time PCR

PMCID: 5788046
PMID: 29372257
DOI: 10.1167/iovs.17-22597

[…] for coding stretches that would yield pcr products with distinct, predicted melting curve profiles. such amplification targets were identified in silico using the melting curve prediction software umelt. predictions were made based on thermodynamic parameters described by blake and delcourt, assuming default concentrations of monovalent ions (20 mm), free magnesium (3 mm), and a temperature […]


A Novel Multiplex HRM Assay to Detect Clopidogrel Resistance

Sci Rep
PMCID: 5700108
PMID: 29167499
DOI: 10.1038/s41598-017-16310-8

[…] development of a multiplex high-resolution melting (hrm) assay to genotype all five of these loci in 2 tubes. amplicons corresponding to distinct snps in a common tube were designed with the aid of umelt prediction software to have different melting temperatures tm by addition of a gc-rich tail to the 5′ end of the certain primers. two kinds of commercial methods, digital fluorescence molecular […]


Derivation of nearest neighbor DNA parameters in magnesium from single molecule experiments

Nucleic Acids Res
PMCID: 5728412
PMID: 29177444
DOI: 10.1093/nar/gkx1161

[…] statistical data analysis allowed us to extract the nnbp energies with 0.1 kcal/mol precision over two orders of magnitude of monovalent salt concentration. these values are currently used by the umelt server to predict melting temperatures. ( ()., force techniques present several advantages compared to bulk methods (e.g. calorimetry, uv absorbance, […]


Reference gene selection for gene expression study in shell gland and spleen of laying hens challenged with infectious bronchitis virus

Sci Rep
PMCID: 5660252
PMID: 29079779
DOI: 10.1038/s41598-017-14693-2

[…] all ten reference genes were selected from the literature published for chickens (table ). specific amplifications of the primers were assessed by generation of a single peak of melting curve using umelt web based tool for predicting dna melting curves and denaturation profiles of pcr products. furthermore, primer specificity was confirmed by obtaining a single band of appropriate size […]

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uMELT institution(s)
School of Computing, University of Utah, Salt Lake City, UT, USA

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