UMIS pipeline

UMIS specifications

Information


Unique identifier OMICS_12783
Name UMIS
Alternative name Unique Molecular IdentifierS
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Python, Shell (Bash)
License Other
Computer skills Advanced
Stability Stable
Source code URL https://codeload.github.com/vals/umis/zip/master
Maintained Yes

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Maintainers


  • person_outline Sarah Teichmann <>
  • person_outline Valentine Svensson <>

Publication for Unique Molecular IdentifierS

UMIS IN pipeline

2016
PMCID: 4927094
PMID: 27355630
DOI: 10.1371/journal.pone.0158195

[…] library. artificial ercc rna spike-in mix 1 (life technologies, usa) was diluted to 1:1000 and 1 μl was used to enable data normalization. in total, 25 pcr cycles were used, but as four base-pair unique molecular identifiers were applied, only the absolute number of unique reads was calculated per analysed sample. the library was sequenced on one lane of illumina hiseq2000, further processed […]

UMIS institution(s)
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, UK; Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK; Centre of Biological Engineering, University of Minho, Braga, Portugal; Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute, Cambridge, UK; Department of Haematology, University of Cambridge, Cambridge, UK
UMIS funding source(s)
Supported by Cancer Research UK grant C45041/A14953; European Research Council project 677501–ZF_Blood; a core support grant from the Wellcome Trust and MRC to the Wellcome Trust–Medical Research Council Cambridge Stem Cell Institute; ERC grant ThSWITCH (grant 260507); a Lister Institute Research Prize; the Wellcome Trust Strategic Award ‘Single cell genomics of mouse gastrulation’.

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