Unblur statistics

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Citations per year

Number of citations per year for the bioinformatics software tool Unblur
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Tool usage distribution map

This map represents all the scientific publications referring to Unblur per scientific context
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Associated diseases

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Popular tool citations

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Protocols

Unblur specifications

Information


Unique identifier OMICS_26911
Name Unblur
Software type Application/Script
Interface Command line interface
Restrictions to use None
Input format MRC/CCP4
Operating system Unix/Linux
Computer skills Advanced
Version 1.0.2
Stability Stable
Maintained Yes

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Documentation


Maintainer


  • person_outline Nikolaus Grigorieff

Publication for Unblur

Unblur citations

 (42)
call_split

Cryo EM of nucleosome core particle interactions in trans

2018
Sci Rep
PMCID: 5935684
PMID: 29728587
DOI: 10.1038/s41598-018-25429-1
call_split See protocol

[…] an image pixel size of 1.4 Å per pixel on the object scale. Data were collected in a defocus range of 10 000–40 000 Å with a total exposure of 100 e/Å2. 40 frames were collected and aligned with the Unblur software package with a dose filter. We collected ~2000 micrographs from several independent NCP preparations. Several thousand particles were manually picked and carefully cleaned in XMIPP to […]

call_split

Cryo EM structure of the RC LH core complex from an early branching photosynthetic prokaryote

2018
Nat Commun
PMCID: 5908803
PMID: 29674684
DOI: 10.1038/s41467-018-03881-x
call_split See protocol

[…] We use Unblur for whole-frame motion correction and exposure weighting, Gctf for estimation of global and local contrast transfer function (CTF) parameters, Gautomatch (developed by Zhang K, MRC Laboratory o […]

call_split

Structural rearrangements of the histone octamer translocate DNA

2018
Nat Commun
PMCID: 5889399
PMID: 29626188
DOI: 10.1038/s41467-018-03677-z
call_split See protocol

[…] image pixel size of 1.4 Å per pixel on the object scale. Data were collected in a defocus range of 10 000–30 000 Å with a total exposure of 100 e Å−2. Forty frames were collected and aligned with the Unblur software package using a dose filter.Several thousand particles were manually picked and carefully cleaned in XMIPP to remove inconsistent particles. The resulting useful particles were used fo […]

library_books

Microscopic charge fluctuations cause minimal contrast loss in cryoEM

2018
Ultramicroscopy
PMCID: 5862660
PMID: 29413413
DOI: 10.1016/j.ultramic.2018.01.011

[…] Fig. 1Fig. 1Fig. 2Fig. 2To calculate quantitatively the image contrast and allow it to be plotted as a function of defocus, as shown in , the frames of each movie were first aligned using the program UNBLUR . Each image was then examined to find bright diffraction beams from gold nanoparticles that were oriented in diffracting positions, as seen with the boxed particle in (a) that has two diffract […]

library_books

Physical basis of amyloid fibril polymorphism

2018
Nat Commun
PMCID: 5816019
PMID: 29453354
DOI: 10.1038/s41467-018-03164-5

[…] The movie frames collected on the K2 Summit were background corrected before motion correction was applied by aligning the frames with the program Unblur. All frames were used in the final frame sums. Furthermore, the images collected on the Titan Krios were corrected for magnification distortion using the program mag_distortion_correct. Fibril […]

call_split

Structure of the Deactive State of Mammalian Respiratory Complex I

2018
PMCID: 5807054
PMID: 29395787
DOI: 10.1016/j.str.2017.12.014
call_split See protocol

[…] Whole-frame alignment was performing using Unblur () before CTF estimation using CTFFIND4 (). All resolution estimates are based on the FSC = 0.143 criterion, and the final resolution estimates were made after the application of a binary mask […]


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Unblur institution(s)
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA; Department of Biochemistry, Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, MA, USA

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