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Associated diseases

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UniPrimer specifications

Information


Unique identifier OMICS_23029
Name UniPrimer
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained No

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Publication for UniPrimer

UniPrimer in publications

 (24)
PMCID: 5532239
PMID: 28751715
DOI: 10.1038/s41598-017-06886-6

[…] primers were designed specifically to socs-3 and socs-4 (table ). to the reverse primer of each pair a z sequence (actgaacctgaccgtaca) was added in order to allow incorporation of the fam tagged uniprimer probe (intergen inc., new york, usa) and subsequent fluorescent detection. an internal standard was run alongside the genes of interest and used to generate a standard curve, allowing […]

PMCID: 5432252
PMID: 28412738
DOI: 10.18632/oncotarget.15439

[…] rna using a reverse transcription kit (biorad, uk). nov mrna expression in crc tissues was determined using real time pcr and an ampliflor™-based pcr method, in which a 6-carboxy-fluorescine-tagged uniprimer™ (biosearch technologies, inc.) was used as a probe together with a pair of target specific primers. the reverse primer contained a z-sequence (actgaacctgaccgtaca) which is complimentary […]

PMCID: 5352358
PMID: 27999191
DOI: 10.18632/oncotarget.13949

[…] (sigma, st. louis, mo, usa). quantitative analysis of nherf1 mrna expression in colorectal tissues was determined by qpcr using amplifor™-based technologies, in which a 6-carboxy-fluorescine-tagged uniprimer™ (biosearch technologies, inc., petaluma, ca, usa) was used as a probe together with a pair of target-specific primers and reverse primer with an additional z-sequence (actgaacctgaccgtaca) […]

PMCID: 5363559
PMID: 27764775
DOI: 10.18632/oncotarget.12729

[…] quantified and subjected to a reverse transcription kit (primerdesign). quantification of the csmd1 and the house keeping actin transcripts were carried using the ampliflor technology in which a uniprimer with a z-sequence (underlined in the following) was used as a probe. the primer sequence for csmd1 and actin were respectively (5′ - 3′): aggagatgagaggccaag vs […]


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UniPrimer institution(s)
Department of Nanobiomedical Science and WCU Research Center, Dankook University, Cheonan, Korea; Department of Clinical Laboratory Science, Juseong University, Cheongwon, Korea
UniPrimer funding source(s)
Supported by the WCU (World Class University) program through the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology (R31-10069).

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