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UTR Designer specifications


Unique identifier OMICS_08703
Name UTR Designer
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline Gyoo Yeol Jung

Publication for UTR Designer

UTR Designer citations


Rapid acquisition and model based analysis of cell free transcription–translation reactions from nonmodel bacteria

Proc Natl Acad Sci U S A
PMCID: 5948957
PMID: 29666238
DOI: 10.1073/pnas.1715806115
call_split See protocol

[…] re 26 RBS-6 variants were transformed and relative RBS activity was determined as a steady-state measurement with cell-free, in vivo, and through in silico prediction, using the RBS Calculator () and UTR Designer () models. Interestingly, two variants (pSJM1211 - 5′-GAGGAGGTATTAT-3′ and pSJM1223 - 5′-AAGGGGGTATTAT-3′) identified from the cell-free screen displayed a twofold enhanced activity in vi […]


Translation efficiency of heterologous proteins is significantly affected by the genetic context of RBS sequences in engineered cyanobacterium Synechocystis sp. PCC 6803

Microb Cell Fact
PMCID: 5834881
PMID: 29499707
DOI: 10.1186/s12934-018-0882-2

[…] Nucleotide sequence analysis of the alternative RBSs in context with the YFP and GFP was performed using the Salis RBS calculator [] and the UTR Designer [] for the prediction of the translation initiation rate, using the − 25 to + 35 sequences of the mRNA transcripts as input (reverse engineering). The same sequences, as well as the entir […]


A set of synthetic versatile genetic control elements for the efficient expression of genes in Actinobacteria

Sci Rep
PMCID: 5765039
PMID: 29323285
DOI: 10.1038/s41598-017-18846-1

[…] umming up, the sequences of the nucleotides located up- and downstream from the SD domain can clearly strongly affect translation efficiency.Figure 1Figure 2There are two widely used online programs (UTR Designer (https://omictools.com/utr-designer-tool) and RBS Calculator (https://www.denovodna.com/software/reverse)) that allow the translation initiation rate from a certain RBS to be predicted,. […]


Oligo and dsDNA mediated genome editing using a tetA dual selection system in Escherichia coli

PLoS One
PMCID: 5515457
PMID: 28719630
DOI: 10.1371/journal.pone.0181501

[…] encodes one of the lycopene synthesis rate-limiting enzymes, in an E. coli strain expressing the λ-Red system on the plasmid pSIM5. A strong 5′-UTR (CTAGTTGGTAAGGAGTCTATAGTTG) was predicted using the UTR designer program []. The same cassette was then replaced with an oligo (i.e., UTR-dxs) containing a strong 5′-UTR flanked by the respective homologous sequences of dxs. Short ssDNA (oligos) transf […]


Mechanisms for Differential Protein Production in Toxin–Antitoxin Systems

PMCID: 5535158
PMID: 28677629
DOI: 10.3390/toxins9070211

[…] equence we used included the entire 5′ UTR region as determined by the transcription start site annotated in the EcoCyc database [] to the end of the TA system. The reverse engineering feature of the UTR Designer was used with the required input of −25 to +35 from the start codon for each gene []. The third method analyzed the sequence −11 to +1 from the start codon using an equation based on empi […]


A simple method to control glycolytic flux for the design of an optimal cell factory

Biotechnol Biofuels
PMCID: 5480111
PMID: 28649279
DOI: 10.1186/s13068-017-0847-4

[…] gene, exhibiting recessive sensitivity to streptomycin in a merodiploid (JHL110). Finally, oligo recombination using [ptsG_UTR(1 to 5)_oligo] that had distinctly redesigned 5′-UTR sequences based on UTR Designer (http://sbi.postech.ac.kr/utr_designer) [] resulted in the ptsG UTR variants, UTR1, 2, 3, 4, and 5, without gaps (Fig. ). The other strains were constructed in the same manner. […]

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UTR Designer institution(s)
Pohang University of Science and Technology, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk, Korea

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