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V-FAT specifications


Unique identifier OMICS_28809
Name V-FAT
Alternative name Viral-Finishing and Annotation Toolkit
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use Academic or non-commercial use
Operating system Unix/Linux
Programming languages Python, R
Computer skills Advanced
Version 1.1
Stability Stable
MUSCLE, Mosaik, GenWise
Registration required Yes
Maintained Yes


No version available


V-FAT citations


Towards development of aptamers that specifically bind to lactate dehydrogenase of Plasmodium falciparum through epitopic targeting

Malar J
PMCID: 5934816
PMID: 29724225
DOI: 10.1186/s12936-018-2336-z

[…] three times with 300 µl of 25 mM Tris-buffered saline, pH 7.6, containing 0.1% (v/v) Nonidet P-40 (NP-40) (Roche, Germany), designated TBS+ hence forth. Wells were then blocked using 100 µl of 2% (w/v) fat-free milk solution in TBS+, incubating for 1 h at 4 °C. Plates were washed three times with 300 µl TBS+. Heat-activated biotinylated oligonucleotides were dissolved in HMCKN to the appropriate […]


Human limbal niche cells are a powerful regenerative source for the prevention of limbal stem cell deficiency in a rabbit model

Sci Rep
PMCID: 5919904
PMID: 29700361
DOI: 10.1038/s41598-018-24862-6

[…] f proteins determined by the BCA assay (Pierce, Rockford, IL) in total cell extracts were separated by 10% SDS-PAGE and transferred to nitrocellulose membranes. Membranes were then blocked with 5% (w/v) fat-free milk in TBST (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% (v/v) Tween-20), followed by sequential incubation with specific primary SCF antibody and their respective secondary antibody using […]


Effect of colorectal cancer derived extracellular vesicles on the immunophenotype and cytokine secretion profile of monocytes and macrophages

PMCID: 5937830
PMID: 29690889
DOI: 10.1186/s12964-018-0229-y

[…] parated by 10% SDS-PAGE. Proteins were electroblotted onto nitrocellulose membranes and stained with Ponceau S solution to check the protein loading. The membranes were destained, blocked with 10% (w/v) fat-free milk and then incubated with the primary antibodies: CD9 (Santa Cruz, Germany) (1:500), ALIX (Santa Cruz, Germany) (1:1000), TSG101 (Abcam, UK) (1:1000), β-actin (Abcam, UK) (1:4000) and C […]


Milk Composition for Admixed Dairy Cattle in Tanzania

Front Genet
PMCID: 5928135
PMID: 29740476
DOI: 10.3389/fgene.2018.00142

[…] tion of the prediction models for the milk traits ranged from 91 to 99%. The parameter estimates for all the milk traits were slightly lower for dilution 2 (16.7% v/v) compared to dilution 1 (33.3% v/v). Fat content exhibited the largest CV; 2.1 and 4.6 for dilution 1 and dilution 2, respectively (Table ). On the other hand, lactose had the small relative variability (CV = 0.77 and 1.23 for diluti […]


Dectin 1/2–induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses

PLoS Biol
PMCID: 5927467
PMID: 29668708
DOI: 10.1371/journal.pbio.2005504

[…] SDS-PAGE, followed by transfer to a PVDF membrane. Membranes were blocked for 1 h at room temperature in TTBS buffer (20 mM Tris–HCl [pH 7.6], 137 mM NaCl, and 0.25% [v/v] Tween 20) containing 5% (w/v) fat-free milk and incubated overnight with primary antibodies. The primary antibodies used were COX-1 (Cell Signalling Technology, Danvers, MA), COX-2 (Cell Signalling Technology), and actin (Merck […]


In Planta Functional Analysis and Subcellular Localization of the Oomycete Pathogen Plasmopara viticola Candidate RXLR Effector Repertoire

Front Plant Sci
PMCID: 5908963
PMID: 29706971
DOI: 10.3389/fpls.2018.00286

[…] e blotted onto a nitrocellulose membrane (PALL, Port Washington, NY, USA) for 1 h at 250 mA, and stained with Ponceau solution to confirm protein loading and transfer. Membranes were blocked in 5% [w/v] fat-free milk in 1 × TBST (0.5% [v/v] Tween-20), after which the primary monoclonal GFP antibody (TransGen) or polyclonal Rubisco large subunit (rbcL) antibody (Agrisera, Vännäs, Sweden) was added […]


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V-FAT institution(s)
Broad Institute, Cambridge, MA, USA

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