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Reverse vaccinology is an emerging vaccine development approach that starts with the prediction of vaccine targets by bioinformatics analysis of microbial genome sequences (Rappuoli, 2000). Predicted proteins are selected based on desirable attributes. Normal wet laboratory experiments are conducted in a later stage to test all or selected vaccine targets.
(Rappuoli, 2000) Reverse vaccinology. Curr Opin Microbiol.
(He et al., 2010) Vaxign: the first web-based vaccine design program for reverse vaccinology and applications for vaccine development. J Biomed Biotechnol.