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Velvet specifications


Unique identifier OMICS_00038
Name Velvet
Software type Package/Module
Interface Command line interface
Restrictions to use None
Biological technology Illumina, Life Technologies, Roche
Operating system Unix/Linux
License GNU General Public License version 2.0
Computer skills Advanced
Version 1.2.10
Stability Stable
Maintained Yes




No version available



  • person_outline Daniel R. Zerbino <>

Publications for Velvet

Velvet citations


The aquatic animals’ transcriptome resource for comparative functional analysis

PMCID: 5954267
PMID: 29764375
DOI: 10.1186/s12864-018-4463-x

[…] had been removed in a previous step, the other read was also removed to synchronize the read pairs., to ensure more complete assembly, we used three de novo assembly tools: oases (v0.2.08, requiring velvet v1.2.09) [, ], soapdenovo-trans (release 1.02) [], and trinity (release 2013–02-25) [] that based on the de bruijn graphs. previous reports suggest that coverage in transcriptomes assembly […]


Complete Sequence of the Intronless Mitochondrial Genome of the Saccharomyces cerevisiae Strain CW252

PMCID: 5920167
PMID: 29700138
DOI: 10.1128/genomeA.00219-18

[…] tophat2 () on the mitochondrial genome of the s. cerevisiae s288c parent strain; they were then sampled by trinity () to obtain an estimated coverage of 30×. an assembly of the genome obtained by velvet () with a k-mer size of 55 produced 34 contigs (n50, 3.7 kb), which were subsequently scaffolded by sspace () to obtain 10 contigs (n50, 57.5 kb). some of these scaffolds seemed […]


Draft Genome Sequence of Multidrug Resistant Escherichia coli NIVEDI P44, Isolated from a Chicken Fecal Sample in Northeast India

PMCID: 5920187
PMID: 29700135
DOI: 10.1128/genomeA.00205-18

[…] sequencing quality control (ngs qc) toolkit version 2.3 () was used to filter high-quality data for the genome assembly. a total of 11,499,584 reads were generated and assembled using the velvet assembler (version 1.2.10) (), yielding 216 contigs of 4,791,462 bp and an n50 value of 90,342 bp. the estimated complete genome size is 4.76 mb, with a g+c content of 50.65%. genome […]


Genome Analysis of Multidrug Resistant Shewanella algae Isolated From Human Soft Tissue Sample

PMCID: 5932639
PMID: 29755354
DOI: 10.3389/fphar.2018.00419

[…] (, quality trimmed with the fastqx-toolkit fastqtrimmer to remove low quality reads ( sequencing data was first assembled using velvet v. 1.2.07 (zerbino and birney, ), and the resulted contigs were then scaffolded with allpaths v. r46652 (butler et al., )., the annotation of the strain yhl was performed using the national […]


Genome comparison of different Zymomonas mobilis strains provides insights on conservation of the evolution

PMCID: 5919020
PMID: 29694430
DOI: 10.1371/journal.pone.0195994

[…] to obtain the best assembled results, we employed two assembly software programs to assemble the genome of z.mobilis, including edena v3 [] and velvet [].these scaffolds were ordered relative to the genome of the z. mobilis strain zm4, using a combination of the contiguator [] and act 10.2.0 []. the illumina reads were remapped […]


Leishmania naiffi and Leishmania guyanensis reference genomes highlight genome structure and gene evolution in the Viannia subgenus

PMCID: 5936940
PMID: 29765675
DOI: 10.1098/rsos.172212
call_split See protocol

[…] and 6 989 814 paired-end l. naiffi lncl223 sequences—85% and 86% of the initial reads, respectively (electronic supplementary material, table s1)., processed reads were assembled into contigs using velvet v1.2.09 and assemblies for all odd-numbered k-mer lengths from 21 to 75 were evaluated. the expected k-mer coverage was determined for each assembly using the mode of a k-mer coverage […]

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Velvet institution(s)
European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK; Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA
Velvet funding source(s)
This work was supported by European Molecular Biology Laboratory’s central funds, and the Intramural Research Program of the National Human Genome Research Institute.

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