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Protocols

ViSP specifications

Information


Unique identifier OMICS_06523
Name ViSP
Software type Package/Module
Interface Command line interface, Graphical user interface
Restrictions to use Academic or non-commercial use
Operating system Mac OS, Windows
Computer skills Advanced
Stability Stable
Maintained No

Versioning


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Publication for ViSP

ViSP citations

 (6)
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Super Resolution Imaging of Genetically Labeled Synapses in Drosophila Brain Tissue

2016
PMCID: 4880563
PMID: 27303270
DOI: 10.3389/fncel.2016.00142
call_split See protocol

[…] ch between glass and the sample in the buffer solution (Huang et al., ) and in ThunderSTORM the signal was adjusted for drift and noise. The three dimensional images were subsequently visualized with ViSP (El Beheiry and Dahan, ). ViSP further provides the possibility to define the surface area and volume of defined objects. […]

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One, two or three? Probing the stoichiometry of membrane proteins by single molecule localization microscopy

2015
Sci Rep
PMCID: 4642553
PMID: 26358640
DOI: 10.1038/srep14072
call_split See protocol

[…] scarded. The majority of peaks were localized with a spatial precision <35 nm according to Mortensen et al. PALM images were generated from the coordinate lists of localized peaks using rapidSTORM or ViSP. Spatio-temporal grouping was applied so that peaks appearing in consecutive camera frames within a spatial separation of 90 nm were registered as a single localization with averaged spatial posi […]

call_split

Drosophila germ granules are structured and contain homotypic mRNA clusters

2015
Nat Commun
PMCID: 4918342
PMID: 26242323
DOI: 10.1038/ncomms8962
call_split See protocol

[…] inforcing the idea that osk is not a stable component of the germ granules.All computations were performed using a custom-written Matlab code (Mathworks), and spatial structures were visualized using ViSP. […]

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Probing the target search of DNA binding proteins in mammalian cells using TetR as model searcher

2015
Nat Commun
PMCID: 4507003
PMID: 26151127
DOI: 10.1038/ncomms8357
call_split See protocol

[…] on laser (). Super-resolution images have been reconstructed with dedicated MatLab routines (available on request), while images rendering and quantification ( and ) have been done using the software ViSP. […]

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Imaging the fate of histone Cse4 reveals de novo replacement in S phase and subsequent stable residence at centromeres

2014
eLife
PMCID: 4067749
PMID: 24844245
DOI: 10.7554/eLife.02203.026
call_split See protocol

[…] events were identified and their 3D coordinates determined with FISHQuant (). Finally, residual 3D drift of the sample was corrected in MatLab (MathWorks, Natick, MA) based on Gold Nanorod fiducials. ViSP software () was used for visualization and presentation of results. Full details for MFM-PALM are available upon request (Hajj et al., unpublished data). […]

library_books

3D imaging of Sox2 enhancer clusters in embryonic stem cells

2014
eLife
PMCID: 4381973
PMID: 25537195
DOI: 10.7554/eLife.04236.035

[…] vents of track fragments which have step and end-to-end displacements less than 50 nm and have lengths longer than the indicated cutoff time. The final 3D image representation was performed by either ViSP () or Imaris. […]

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