VIPER specifications


Unique identifier OMICS_18454
Alternative name Visual Inspection of Peak/Elution Relationships
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input format CSV, mzXML, mzData
Operating system Windows
Computer skills Medium
Version 3.49
Stability Stable
Source code URL
Maintained Yes



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  • person_outline Richard Smith <>

VIPER article

VIPER citations

PMCID: 4951065

[…] in the technical replicates. isotopic features were combined across ms scans to provide lc-ms elution features for comparison back to the look-up table derived from ms/ms identifications using viper [30]., for presence versus absence analysis, proteins represented by a single peptide were first removed. then, to add confidence to the presence of a protein within a sampled time point, […]

PMCID: 4286152

[…] alignment of mass and the lc elution time make it possible to narrow the mass tolerance used in generating xics and the elution time window for selecting the correct chromatographic peak. currently, viper29 is used in a first-pass analysis (using conventional accurate mass and time, or amt tag approach) to output mass and normalized elution time (net) alignment information, which is then loaded […]

PMCID: 3981673

[…] and fragment ion tolerances, respectively. high resolution lc-ms features were deconvoluted by decon2ls (version 1.0.2, using default parameters) and aligned to the mass and time tag database using viper (version 3.45 using default parameters) using the theoretical mass and observed normalized elution times (net) for each peptide. this approach to proteomics research is enabled by a number […]

VIPER institution(s)
Pacific Northwest National Laboratory, Richland, WA, USA
VIPER funding source(s)
Supported by the US Department of Energy Office of Biological and Environmental Research Genomes:GtL Program, the NIH National Center for Research Resources (Grant RR018522) and the National Institute of Allergy and Infectious Diseases (NIH/DHHS through interagency agreement Y1-AI-4894-01).

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