Volocity protocols

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Volocity specifications

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Unique identifier OMICS_06504
Name Volocity
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use License purchase required
Operating system Mac OS, Windows
License Commercial
Computer skills Advanced
Stability Stable
Maintained No

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Volocity in pipelines

 (39)
2018
PMCID: 5778106
PMID: 29403481
DOI: 10.3389/fimmu.2017.02001

[…] and rat anti-endomucin (santa cruz biotechnology). nuclei were labeled using dapi (sigma). images were acquired using a zeiss lsm 780 confocal microscope and analyzed using imaris (bitplane), volocity (perkin elmer), and imagej (nih). one to four slices were randomly selected from at least three animals per group. for quantification of infiltrated islets, four slices were randomly […]

2018
PMCID: 5834689
PMID: 29440406
DOI: 10.1073/pnas.1716266115

[…] and lasers exciting at 405 nm (41.5 mw), 488 nm (21.2 mw), and 561 nm (20.2 mw). image analysis was conducted using metamorph imaging series 7 software (universal imaging), imaris 7 (bitplane), or volocity (perkinelmer). images are presented as either single planes or projections, as stated in legends. we used an unbiased blinded approach for the analysis of scoring protein labeling at the is. […]

2017
PMCID: 5356445
PMID: 28148823
DOI: 10.1098/rsob.160312

[…] 28.5°c. we used sp5 (leica), lsm 880 (zeiss), lsm 710 (zeiss) laser scanning or spinning disk (perkinelmer) confocal microscopes. for image data analysis, we used imagej (http://rsbweb.nih.gov/ij/), volocity (perkinelmer) or imaris (bitplane) software., all statistical analyses were performed using prism7 (graphpad software). to compare the differences in number of apical and non-apical […]

2017
PMCID: 5425629
PMID: 28416284
DOI: 10.1016/j.stemcr.2017.03.016

[…] hpf), runx1+144:gfp+/flk1:dsred+ embryos were embedded in 0.8%–2% low-melting agarose with tricaine (sigma) and mounted on 35-mm center-glass dishes. image analysis was performed using imagej (nih), volocity (perkinelmer), or imaris (bitplane) software., runx1+144:gfp+ embryos were incubated at 72 hpf on ice for 10 min, followed by a 2-hr incubation in 400 μm edu (4% dmso/e3 buffer) at 28°c. […]

2017
PMCID: 5540285
PMID: 28767724
DOI: 10.1371/journal.pone.0181579

[…] equipped with a 40xp-neofluar (na 1.3) and 63x plan-apochromat objective (na1.4) were used. images were acquired using the zeiss software (aim4). three dimensional reconstructions were made using volocity software (perkin elmer) and imaris scientific 3d/4d image processing analysis software (bitplane ag, zurich, switzerland). images analyses were performed using imagej software and lsm image […]

Volocity in publications

 (2894)
PMCID: 5954120
PMID: 29765094
DOI: 10.1038/s41598-018-25882-y

[…] incubated for 1 h with a 1:200 dilution of cy2-coupled anti-mouse igg in pbs-triton-bsa. following washing, slides were mounted with 5% (wt/vol) polyvinyl alcohol., images were acquired using the volocity software package. image analysis was performed using the microbetracker matlab software. distributions of cell dimensions among cell populations were represented with box plots displaying […]

PMCID: 5951861
PMID: 29760469
DOI: 10.1038/s41467-018-04288-4

[…] containing stained cells were set in a humidity chamber with a temperature-control system to allow flagellar growth at 30 °c. time-lapse fluorescence images were captured at 10-min intervals using volocity 3d image analysis software running on a pc laptop. a 1.2 µm z-axis scan, with images taken every 0.2 µm for each layer, was adopted to obtain high quality images of the bacterial flagella., […]

PMCID: 5945782
PMID: 29748644
DOI: 10.1038/s41598-018-25698-w

[…] time of 50 msec and 100 msec, gain of 100% and 100%, range of 150 μm and 150 μm, slices of 31 and 31, respectively. the 2d/3d images were constructed by imagej/fiji image analysis platform or volocity software (perkinelmer, inc. massachusetts, usa) (figs  and , and supplementary movie ). the 2d/3d images were retrospectively analysed at several points (fig. )., all statistical analyses […]

PMCID: 5940834
PMID: 29740074
DOI: 10.1038/s41598-018-25606-2

[…] neuropeptide precursor arrgpp. images of stained sections were captured using a dmra2 light microscope (leica) with a micropublisher 5.0 real-time viewing (rtv) digital colour camera (qimaging) and volocity® v.5.3.1 image analysis software (perkinelmer). montages of photographs were prepared using the software adobe photoshop cc 2015., informed by findings from analysis of the expression […]

PMCID: 5933757
PMID: 29723289
DOI: 10.1371/journal.pone.0196954

[…] with e. coli strain op50 or transferred to assay plates for 1 hr prior to imaging. images (0.4 μm z-stacks) were captured using olympus fluoview fv1000 software and fluorescence was quantified using volocity software version 4.0.0 build 314 (improvision ltd). to quantify neuropeptide fluorescence, both the right and left asis were tightly cropped and divided into the soma and axon. all images […]

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