Recognizes all potential specific single-copy and/ or repetitive Fluorescence in-situ hybridization (FISH) probes inside a user-specified chromosomal sequence. webFISH can design FISH probes for complicated targets. It can be used to test potential FISH probes derived from stock plasmids for their specificity to a single genomic site and thereby guide the selection of the most suitable clones. The tool’s features can be applied to RNA.
Randall Division of Cell and Molecular Biophysics, King’s College London, London, UK; MRC and Asthma UK Centre in Allergic Mechanisms of Asthma, Guy’s Hospital, London, UK; Division of Asthma, Allergy and Lung Biology, Guy’s Hospital, London, UK; Institute of Photonics Technology, Jena, Germany; Institute of Physical Chemistry, Friedrich Schiller University, Jena, Germany
webFISH funding source(s)
Supported by a Medical Research Council and Asthma UK Centre in Allergic Mechanisms of Asthma PhD studentship, by the Medical Research Council [grant G0501494]; by Biotechnology and Biological Sciences Research Council [grant BB/H019634/1]; and by Engineering and Physical Sciences Research Council [grant EP/F069731/1]; by the Department of Health via the National Institute for Health Research comprehensive Biomedical Research Centre award to Guy’s St Thomas’ National Health Service Foundation Trust in partnership with King’s College London and King’s College Hospital National Health Service Foundation Trust.