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A software package for automatic phenotyping in C. elegans included in QuantWorm. WormGender is designed for accurate quantification of sex ratio in Caenorhabditis elegans. The software functions include, i) automatic recognition and counting of adult hermaphrodites and males, ii) a manual inspection feature that enables manual correction of errors, and iii) flexibility to use new training images to optimize the software for different imaging conditions. We evaluated the performance of our software by comparing manual and automated assessment of sex ratio. Our data showed that the WormGender software provided overall accurate sex ratio measurements.

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WormGender classification

  • Animals
    • Caenorhabditis elegans

WormGender specifications

Software type:
Restrictions to use:
Academic or non-commercial use
Programming languages:
Computer skills:
Graphical user interface
Operating system:
Mac OS, Windows
Academic Free License version 3.0

WormGender distribution


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WormGender support



  • Weiwei Zhong <>


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Department of BioSciences, Rice University, Houston, TX, USA

Funding source(s)

This research was funded by the National Institutes of Health grants (HG004724 and DA018341) and by a Searle Scholar grant.

Link to literature

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