WU-CRISPR statistics

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Citations per year

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Popular tool citations

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Tool usage distribution map

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Associated diseases

Associated diseases

WU-CRISPR specifications


Unique identifier OMICS_10078
Interface Web user interface
Restrictions to use None
Input data One sequence at a time, consisting of A, T or U, C and G
Computer skills Basic
Stability Stable
Maintained Yes


  • Primates
    • Homo sapiens
  • Rodents
    • Mus musculus


  • person_outline Xiaowei Wang <>

Publication for WU-CRISPR

WU-CRISPR in publications

PMCID: 5519727
PMID: 28729655
DOI: 10.1038/s41598-017-06269-x

[…] were examined under ix51fluorescence microscope of olympus (olympus, tokyo, japan)., hiv provirus targeting sites for crispr/cas9 system were designed by using a free online bioinformatics software wu-crispr (http://crispr.wustl.edu/cgi-bin/grna_predict/custom_grna.cgi). the off-target effects of grnas against human genome were investigated by another free online bioinformatics software […]

PMCID: 4991477
PMID: 26942882
DOI: 10.18632/oncotarget.7828

[…] pcr for cloning purpose. dual grna targeting the entire exon of linc00657 and the corresponding donor were constructed using the same method as described previously []. dual grna was designed using wu-crispr [] and their sequences were listed in . to increase the frequency of selection of complete knockout clones, we constructed a donor vector carrying left and right arm homologous […]

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WU-CRISPR institution(s)
Department of Biomedical Engineering, Washington University, St Louis, MO, USA; Department of Radiation Oncology, Washington University School of Medicine, St Louis, MO, USA
WU-CRISPR funding source(s)
This work was supported by the National Institutes of Health [R01GM089784].

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