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xCell specifications

Information


Unique identifier OMICS_16733
Name xCell
Interface Web user interface
Restrictions to use None
Input data The gene expression data.
Input format TXT, CSV
Output data The cell type enrichment scores.
Programming languages R
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Dvir Aran <>

Publication for xCell

xCell in pipelines

 (3)
2015
PMCID: 4382096
PMID: 25830507
DOI: 10.1371/journal.ppat.1004793

[…] version 2.17.0 []., cell lysates were separated by sds-page on 4–12% or 12% bis-tris nupage gels in mes or mops running buffer (life technologies). either the iblot dry blotting system or the xcell ii blot module (life technologies) was used to transfer proteins to either pvdf or nitrocellulose. membranes were blocked with superblock blocking buffer (pierce) with 0.25% surfact-amps 20 […]

2013
PMCID: 3681718
PMID: 23785293
DOI: 10.1371/journal.pgen.1003272

[…] −) and nickel affinity columns. chromatography fractions were submitted tor 1d sds-gel electrophoresis (nupage electrophoresis system, invitrogen) and then transferred to nitrocellulose membranes (xcell ii blot module, invitrogen). membranes were blocked with milk-pbs-t (0.02% v/v tween, 10% dry milk) for 20 min. anti-(his)5 hrp conjugate (qiagen) was added to a final dilution of 1∶20,000 […]

2010
PMCID: 2893103
PMID: 20550709
DOI: 10.1186/1476-4598-9-149

[…] -80°c. ten micrograms of cell line lysate and 10 nanograms of ragr2 or ragr3 were separated using nupage novex 4%-12% bis-tris gels at 200v for 40 minutes and transferred to nitrocellulose using the xcell surelock mini-cell (invitrogen) at 30v for 60 minutes. membranes were blocked overnight in blocking buffer (50 mm tris; 150 mm nacl; 5% skim milk powder). membranes were then incubated with 2 […]


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xCell in publications

 (1286)
PMCID: 5952831
PMID: 29764418
DOI: 10.1186/s12934-018-0924-9

[…] washed twice with 50 ml of sterile water. the cell pellet was finally resuspended and aliquoted (50 µl each) in sterile 20% glycerol. plasmids were transformed by electroporation using gene pulser xcell™ electroporation system (bio-rad laboratories, inc. hercules, california, usa). 100 ng of dna were added to 50 µl electrocompetent cells and transferred to a 0.2 cm electroporation cuvette. […]

PMCID: 5943249
PMID: 29743487
DOI: 10.1038/s41598-018-23884-4

[…] cells were transfected with the same total amount of dna by supplementing with empty vector. cells were incubated for 10 min at rt with the dna mix and electroporated using the gene pulser xcell™ electroporation system (bio-rad) at 260 mv, 960 mf in 400 µl of rpmi 1640. expression of the different proteins was confirmed by western blot. the transfection efficiency in jurkat tag cells […]

PMCID: 5940819
PMID: 29740007
DOI: 10.1038/s41598-018-24999-4

[…] into a ptv00 digested with bamh i and hind iii for generating ptv00::miise5. the constructs were then transformed into the agrobacterium tumefaciens gv3101 using electroporation (bio_rad gene pulser xcell®, usa). the pepper plants were inoculated by gv3101 carrying the corresponding constructs using procedures as previously described,, the infiltrated pepper plants were inoculated with 800 m. […]

PMCID: 5955458
PMID: 29726306
DOI: 10.1080/21505594.2018.1451284

[…] expression vector ppiczαa in frame with a poly-histidine tag added to the c-terminal. the plasmids were linearized by sac i or bstx i and introduced into p. pastoris by electroporation (genepulser xcell, bio-rad) according to the provider's protocol. transfectants were screened by growth on ypd + 200 µg/ml of zeocin (thermo fisher scientific, cat no. r25005) and confirmed by sanger […]

PMCID: 5933787
PMID: 29723287
DOI: 10.1371/journal.pone.0197012

[…] cold 250 mm sucrose, resuspended in 50 μl cold 250 mm sucrose, mixed with 1 μg of pmw1650 plasmid, placed in a 0.1 cm cuvette, and electroporated at 1.8 kv, 200 ohms, 25 μf, 5 ms using a gene pulser xcell (bio-rad). bacteria were immediately recovered in 1.2 ml brain heart infusion (bhi) medium. for infections of confluent vero cells in 6-well plates, medium was removed from each well, and cells […]


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xCell institution(s)
Institute for Computational Health Sciences, University of California, San Francisco, CA, USA
xCell funding source(s)
This work was supported by a Gruss Lipper Postdoctoral Fellowship, the National Cancer Institute (U24 CA195858) and the National Institute of Allergy and Infectious Diseases (Bioinformatics Support Contract HHSN272201200028C).

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