Protocols

xComb specifications

Information


Unique identifier OMICS_27503
Name xComb
Restrictions to use None
Community driven No
Data access Browse
User data submission Not allowed
Maintained No

Maintainer


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Publication for xComb

xComb citations

 (2)
library_books

Structural Basis of Thermal Stability of the Tungsten Cofactor Synthesis Protein MoaB from Pyrococcus furiosus

2014
PLoS One
PMCID: 3896444
PMID: 24465852
DOI: 10.1371/journal.pone.0086030

[…] fragmented in the linear ion trap using collision-induced dissociation (CID). Databases containing cross-linked peptides were generated for PfuMoaB-WT and the H3 variant using the publicly available xComb software (version 1.3) with the following settings: enzyme specificity trypsin, two missed cleavages, intra- and inter-molecular cross-links, type of cross-linker used EDC, minimum peptide lengt […]

call_split

A Mechanism for Actin Filament Severing by Malaria Parasite Actin Depolymerizing Factor 1 via a Low Affinity Binding Interface*

2013
PMCID: 3924271
PMID: 24371134
DOI: 10.1074/jbc.M113.523365
call_split See protocol

[…] es consisting of full-scan MS, low-resolution MS/MS (OrbiTrap XL), and high-resolution MS/MS spectra (Q-Exactive) were converted to the MGF data format with Proteome Discoverer 1.4 and searched using Xcomb generated databases with the Mascot or pLink algorithms, respectively (). Databases for every possible cross-linked pair were generated by xComb version 1.3 (). The amino acid sequences for the […]

xComb institution(s)
Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA
xComb funding source(s)
Supported by the National Institutes of Health (NIH): R33CA099139-01, 1S10RR023044-01 and 1U54 AI57141-01; and the Swiss National Science Foundation (SNF) (PBLAA—119623 and PA00P3_126252).

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