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Protocols

XPRESS specifications

Information


Unique identifier OMICS_02488
Name XPRESS
Alternative name TPP distribution
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Maintained Yes

Versioning


No version available

Maintainer


  • person_outline Ruedi Aebersold

Publication for XPRESS

XPRESS citations

 (1281)
library_books

Mutation analysis of BRCA1/2 mutations with special reference to polymorphic SNPs in Indian breast cancer patients

2018
PMCID: 5953318
PMID: 29785135
DOI: 10.2147/TACG.S155955

[…] primer sequences and phosphorylate the amplicons at 50°c for 10 min, followed by 55°c for 10 min, then 60°c for 20 min. the amplicons were ligated to adapters with the diluted barcodes of the ion xpress™ barcode adapters kit (thermo fisher scientific) for 30 min at 22°c, then 72°c for 10 min. adaptor-ligated amplicon libraries were purified using agencourt ampure xp (cat no. a63881) reagents […]

library_books

Plastid genome analysis of three Nemaliophycidae red algal species suggests environmental adaptation for iron limited habitats

2018
PLoS One
PMCID: 5940233
PMID: 29738547
DOI: 10.1371/journal.pone.0196995

[…] were generated using the ion torrent pgm (thermo fisher scientific, san francisco, california, usa) next-generation sequencing (ngs) platform. the sequencing libraries were prepared using the ion xpress plus gdna fragment library preparation kit for 200 bp or 400 bp libraries. the library amplification and dna sequencing were conducted by either ion pgm template ot2 200 or 400 kits and ion […]

library_books

Whole genome sequencing in the search for genes associated with the control of SIV infection in the Mauritian macaque model

2018
Sci Rep
PMCID: 5940699
PMID: 29739964
DOI: 10.1038/s41598-018-25071-x

[…] in the intermediate pvl group. this procedure captured 424 variants distributed in 279 genes (supplementary table )., barcoded-library dna samples from the 42 animals were prepared with an ion xpress plus fragment library kit and ion xpress barcode adaptors 1–96 kit according to the manufacture’s protocol for 200 base-read sequencing (life technologies/thermo fisher scientific). five […]

library_books

Draft Genome Sequence of Burkholderia cepacia ATCC 17759, a Polyhydroxybutyrate Co Valerate Copolymer Producing Bacterium

2018
Genome Announc
PMCID: 5920182
PMID: 29700161
DOI: 10.1128/genomeA.00348-18

[…] purification kit (promega). dna quality and concentration were determined by gel electrophoresis and spectrophotometry (nanodrop, thermo scientific). a dna library was prepared using the ion torrent xpress plus fragment library kit (400 bp), templated using the ion torrent ot2 400 template kit, and sequenced on the ion torrent pgm platform using the ion torrent pgm 400 sequencing kit (thermo […]

library_books

Mutational profiling can identify laryngeal dysplasia at risk of progression to invasive carcinoma

2018
Sci Rep
PMCID: 5919930
PMID: 29700339
DOI: 10.1038/s41598-018-24780-7

[…] scientific) as described by the manufacturer. dna from pd1 and pd5 and their associated lscc (lscc1 and lscc5) were sequenced as a pool. for each sample, 100-ng of dna was barcoded using the ion xpress barcode adapter kit and multiplexed for emulsion pcr. the resulting amplicons were sequenced using the ion sequencing kit v. 2.0 and an ion 316 chip (8 samples/chip) on an ion torrent personal […]

library_books

A Novel Whole Gene Deletion of BCKDHB by Alu Mediated Non allelic Recombination in a Chinese Patient With Maple Syrup Urine Disease

2018
Front Genet
PMCID: 5928131
PMID: 29740478
DOI: 10.3389/fgene.2018.00145

[…] used for pcr enrichment of target genes using ion ampliseq custom panel (life technologies). the amplification library of the target exons was prepared using an ion ampliseq library kit 2.0 and ion xpress barcode adapter 1-16 kit (life technologies). the emulsion pcr was performed using the ion onetouchtm system and ion pgmtm hi-qtm view ot2 kit (life technologies). then, template-positive ion […]


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XPRESS institution(s)
University of Connecticut Health Center, Farmington, CT, USA; Institute for Systems Biology, Seattle, WA, USA

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